FIGURE

Fig. 3.

ID

ZDB-FIG-241218-84

Publication

Siddiqui et al., 2024 - Stat3 mediates Fyn kinase-driven dopaminergic neurodegeneration and microglia activation

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Fig. 3.

Zebrafish neural FynY531F overexpression drives microglia activation and cytokine induction. (A) 4C4 immunolabeling of microglia in wild-type +/+ and elavl3:Gal4; UAS:fynY531F forebrain and midbrain (MB)/hindbrain (HB). (B) Quantification of 4C4-labeled microglia in 5 dpf +/+ and elavl3:Gal4; UAS:fynY531F larval brain (n=12). (C) Higher-magnification images of 4C4-labeled microglia in +/+ and elavl3:Gal4; UAS:fynY531F larvae. Yellow circles outline microglia with ramified morphology; yellow ovals outline microglia with intermediate or amoeboid morphology. (D) Quantification of microglia with ramified, intermediate and amoeboid morphologies in 5 dpf +/+ and elavl3:Gal4; UAS:fynY531F larval brain (n=4). (E) Reverse transcription quantitative PCR (RT-qPCR) of il1b, il12a, tnfa and irg1 in RNA extracts from control elavl3:Gal4 and elavl3:Gal4; UAS:fynY531F 5 dpf larvae (n=3 biological replicates for each genotype). Statistical analysis was performed with two-tailed unpaired Student’s t-test. Bars represent mean±s.e.m. ns, not significant; *P<0.05; **P<0.01. Scale bars: 50 µm (A); 20 µm (C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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