FIGURE

Fig. 1.

ID

ZDB-FIG-241218-82

Publication

Siddiqui et al., 2024 - Stat3 mediates Fyn kinase-driven dopaminergic neurodegeneration and microglia activation

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Fig. 1.

Gal4-UAS overexpression of FynY531F leads to autophosphorylation of Y416, trunk curvature and swimming defects. (A) Diagram of elavl3:Gal4; 14XUAS:fynY531F neural-specific model. (B) Western blot probed with anti-P-Y416-SFK antibody, showing elevated Y416 phosphorylation in 5 days post fertilization (dpf) elavl3:Gal4; 14XUAS:fynY531Fis89 and elavl3:Gal4; 14XUAS:fynY531Fis90 in comparison to wild-type +/+ larvae. Anti-β-actin antibody was used as a loading control. (C) Trunk curvature observed in a subset of 5 dpf elavl3:Gal4; 14XUAS:fynY531Fis90 larvae. Plot shows the percentages of 5 dpf wild-type +/+, elavl3:Gal4; 14XUAS:fynY531Fis89 and elavl3:Gal4; 14XUAS:fynY531Fis90 larvae showing trunk curvature. (D) Measurements of velocity (left) and distance moved (middle) in 5 dpf wild-type +/+ and elavl3:Gal4; 14XUAS:fynY531Fis90 larvae that showed normal trunk morphology. Circular plots (right) show 5 s snapshots of tracking movements. Normal movements (green lines) and large movements (red lines) are shown for a wild-type 5 dpf +/+ larva and an elavl3:Gal4; 14XUAS:fynY531Fis90 larva with normal trunk morphology. Scale bars: 500 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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