Fig. 4.

ELC alters GC_R to a second vortex during the refractory period. (A) Sampling scheme for measuring GC_R to homotypic stress in 6 dpf larvae with or without ELC. (B) Whole-body cortisol following two 3-min vortices applied within a 30-min refractory period in control (black) and ELC (orange) larvae. Control larvae did not show cortisol levels above baseline in response to a second 3-min vortex, while ELC larvae did. N=6 per group. ****P<0.0001 by unpaired two-tailed t-test. (C) Baseline whole-body cortisol levels in ELC larvae relative to controls (data from Fig. 1C), depicted for the sake of interpreting panels D and E. P=0.40 by one-sample t-test against fold change of ‘1′. (D) GC_R to 1st and 2nd vortices relative to baseline in control and ELC larvae. Data from Fig. 1C,D (10 min post-vortex), and B. N=6 per group. P-values indicate Bonferroni's test results following a two-way ANOVA. (E) GC_R to the 2nd vortex relative to GC_R to the 1st vortex in control (black) and ELC (orange) larvae (data from D). P<0.0001 by unpaired two-tailed t-test. (B-E) Box and whiskers (min. to max.), (C-E) all data points shown.