|
Forced swimming at 5 dpf reduces a larva's cortisol response to a subsequent exposure to the same type of stress. (A,B) Comparison steps and time points for measuring baseline whole-body cortisol levels (A) and whole-body cortisol trajectory post-vortex (B) in 6 dpf larvae with or without ELC exposure. (C,D) Baseline whole-body cortisol levels (C) and whole-body cortisol trajectory post-vortex (D) in control (black) and ELC (orange) larvae. (C) P=0.90 by Mann–Whitney test; N=6 per group. Box and whiskers (min. to max.), all data points shown. (D) 10′ and 20′: ****P<0.0001, 30′: **P=0.003, 40′: P=0.125, 60′: P=0.99 by Bonferroni's test after a two-way ANOVA on square root-transformed cortisol levels; N=6 per group. Mean±s.e.m. The red line represents the average baseline whole-body cortisol levels from C.
|