Expression of EGFP by hoxa13begfp and hoxd13aegfp in pectoral fin development of zebrafish. (A-F) EGFP expression induced by hoxa13begfp in pectoral fin development of WT (A-C) and hhip−/− (D-F) zebrafish. (G,H) EGFP expression induced by hoxd13aegfp in pectoral fin development of WT. Numbers indicate the first to fourth proximal radials. White arrowheads indicate significant expression of EGFP by hoxa13begfp. White bars represent the range of EGFP expression. White asterisks denote EGFP expression by col2a1a:egfp on the distal radials. Observations were conducted on more than five larvae at each developmental stage. Magenta fluorescence indicates the paired fin skeleton marked by sox10:DsRed. Double arrows indicate the anterior (A)-posterior (P) axis. The standard length of individuals (in mm) is shown in the top right of each panel. Scale bars: 250 µm.
|