FIGURE

Fig. 1

ID

ZDB-FIG-241119-18

Publication

Zhang et al., 2024 - Cytosine base editors with increased PAM and deaminase motif flexibility for gene editing in zebrafish

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Fig. 1

Cytosine base editing in all sequence contexts mediated by zevoCDA1-BE4max.a Schematic of the mRNA construct for zevoCDA1-BE4max. bpNLS: bipartite nuclear localization, zevoCDA1: cytosine deaminase, XTEN: a 32aa flexible linker, nSpCas9: SpCas9 nickase, linker: SGGSSGGS amino acid, UGI: Uracil glycosylase inhibitor. b Comparison of the editing efficiency of zAncBE4max, evoCDA1-BE4max, and zevoCDA1-BE4max, targeting six loci with NGG PAM. The data represent the aggregate result of three independently replicated experiments, and the error bars indicate the standard deviation of the mean values. Target sequence information is displayed below the data, respectively. c Summary of editing efficiency of zevoCDA1-BE4max at 21 NGG PAM sites on 20 genes. d?f Sanger sequencing results of zevoCDA1-BE4max at 3 loci. The sequence and name of gRNAs are labelled above the Sanger results. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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