ZFIN Image: Zhang et al., 2024, Fig. 1

Image

Figure Caption

Fig. 1 Cytosine base editing in all sequence contexts mediated by zevoCDA1-BE4max.a Schematic of the mRNA construct for zevoCDA1-BE4max. bpNLS: bipartite nuclear localization, zevoCDA1: cytosine deaminase, XTEN: a 32aa flexible linker, nSpCas9: SpCas9 nickase, linker: SGGSSGGS amino acid, UGI: Uracil glycosylase inhibitor. b Comparison of the editing efficiency of zAncBE4max, evoCDA1-BE4max, and zevoCDA1-BE4max, targeting six loci with NGG PAM. The data represent the aggregate result of three independently replicated experiments, and the error bars indicate the standard deviation of the mean values. Target sequence information is displayed below the data, respectively. c Summary of editing efficiency of zevoCDA1-BE4max at 21 NGG PAM sites on 20 genes. d?f Sanger sequencing results of zevoCDA1-BE4max at 3 loci. The sequence and name of gRNAs are labelled above the Sanger results. Source data are provided as a Source Data file.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.