FIGURE

Figure 1.

ID

ZDB-FIG-241017-36

Publication

Ree et al., 2024 - Naa80 is required for actin N-terminal acetylation and normal hearing in zebrafish

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Figure 1.

Actin N-terminal maturation scheme and the in vitro acetyltransferase activity of Danio rerio E7FBQ5/Naa80 towards actin-type N-termini.

(A) Overview of actin N-terminal processing leading up to Nt-acetylation. Class I actins are first co-translationally Nt-acetylated by NatB, and then actin methionine aminopeptidase removes the acetylated Met, before NAA80 acts on the acidic neo-N-terminus. Class II actins are processed by MetAP1/2 before Nt-acetylation by NatA and removal of an acetylated Cys by actin methionine aminopeptidase before Nt-acetylation by NAA80. In both humans and zebrafish, actin is conserved with most of the amino acid differences located to the N-terminus. (B) Acetylation assays with purified MBP- E7FBQ5/zNaa80 and different peptides derived from human proteins. (C) Acetylation assays with purified MBP- E7FBQ5/zNaa80 and different peptides derived from zebrafish actins.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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