PUBLICATION
Naa80 is required for actin N-terminal acetylation and normal hearing in zebrafish
- Authors
- Ree, R., Lin, S.J., Sti Dahl, L.O., Huang, K., Petree, C., Varshney, G.K., Arnesen, T.
- ID
- ZDB-PUB-241011-11
- Date
- 2024
- Source
- Life science alliance 7(12): (Journal)
- Registered Authors
- Lin, Sheng-Jia, Varshney, Gaurav
- Keywords
- none
- MeSH Terms
-
- Actins*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Zebrafish*/metabolism
- Hearing/physiology
- Humans
- Animals
- Acetyltransferases/genetics
- Acetyltransferases/metabolism
- Acetylation
- PubMed
- 39384430 Full text @ Life Sci Alliance
Citation
Ree, R., Lin, S.J., Sti Dahl, L.O., Huang, K., Petree, C., Varshney, G.K., Arnesen, T. (2024) Naa80 is required for actin N-terminal acetylation and normal hearing in zebrafish. Life science alliance. 7(12):.
Abstract
Actin is a critical component of the eukaryotic cytoskeleton. In animals, actins undergo unique N-terminal processing by dedicated enzymes resulting in mature acidic and acetylated forms. The final step, N-terminal acetylation, is catalyzed by NAA80 in humans. N-terminal acetylation of actin is crucial for maintaining normal cytoskeletal dynamics and cell motility in human cell lines. However, the physiological impact of actin N-terminal acetylation remains to be fully understood. We developed a zebrafish naa80 knockout model and demonstrated that Naa80 acetylates both muscle and non-muscle actins in vivo. Assays with purified Naa80 revealed a preference for acetylating actin N-termini. Zebrafish lacking actin N-terminal acetylation exhibited normal development, morphology, and behavior. In contrast, humans with pathogenic actin variants can present with hypotonia and hearing impairment. Whereas zebrafish lacking naa80 showed no obvious muscle defects or abnormalities, we observed abnormal inner ear development, small otoliths, and impaired response to sound. In conclusion, we have established that zebrafish Naa80 N-terminally acetylates actins in vitro and in vivo, and that actin N-terminal acetylation is essential for normal hearing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping