Fig. 2

DLD-dependent enzyme metabolite analysis and mitochondrial function in dldh?/? zebrafish larvae. (A?H) Biochemical analysis by GC/MS of DLD-dependent enzyme metabolite and aa, relative to undecanoic acid standard and normalized to protein ?-ketoglutarate, valine, isoleucine, leucine, lysine, glutarate, ?-ketoisovalerate, and lactate in 7 dpf WT (dldh+/+ and dldh+/?)and dldh?/? zebrafish larvae. n = 5 biological replicates/condition. ***P < 0.001; ****P < 0.0001; Welch?s unpaired t test. Data are shown as mean ± SD. (I?K) Lactate and pyruvate, analyzed using lactate and pyruvate oxidase-based colorimetric assays, in 7 dpf dldh?/? zebrafish larvae. Data were normalized to WT sibling controls. *P < 0.05. Student?s t test. (L and M) Analysis of NADH and GSH and GSSG levels (percentage of control) showed no differences between dldh?/? and WT larvae at 7 dpf. All colorimetric analyses were conducted on 4 WT and 3 dldh?/? larval biological replicate samples. Data are shown as mean ± SD.