FIGURE

Figure 4

ID

ZDB-FIG-240930-142

Publication

Trengove et al., 2024 - Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling

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Figure 4

Analysis of the impact of zebrafish Socs4b on EGFR signaling. HEK293T cells transfected with empty vector control or plasmids encoding Flag-tagged Socs4b WT or Socs4b Δ18 were stimulated with EGF for the time indicated (min). Protein was extracted and subjected to Western blot analysis with antibodies against the Flag-tag to confirm expression of the Socs4b forms, as well as to pEGFR and pERK to detect EGFR signaling, along with tERK and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) that served as loading controls. The position of Flag-tagged Socs4b WT and Socs4b Δ18 proteins are shown with blue and purple arrowheads, respectively. Bands were quantified and the relative ratio of pEGFR:GAPDH and pERK:tERK calculated and shown below the relevant panel, with the 0 min sample set at 1.0 in each case, showing the average of two independent replicates. Original blots can be found in Supplementary Figure S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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