Fig. 4

ccdc141 is required for asymmetry decay of dand5 and normal KV flow. A and B: The correct expression (right) and aberrant expression (left, bilateral) of dand5 at 8 ss are shown. Control vs. crispants, N = 3; n = 34/63; ????, P < 0.0001; two-sided Fisher's exact test. C and D: Representative DIC images (C) and qualification (D) of different KVs in control and crispants embryos at 8 ss. Control vs. crispants, n = 30/10; ??, P < 0.01. E?G: Knockdown of ccdc141 decreases the cilia number per KV, shorter the cilia length. Cilia number, n = 11/14 embryos. ???, P < 0.001; two-sided unpaired t-test. Cilia length, N = 11/14; n (cilia) = 293/282. ????, P < 0.0001; Mann-Whiteney test. H and I: The kymograph and the quantification of cilia beating. Control vs. crispants, N = 9/10, n (cilia) = 158/124. ????, P < 0.0001; Mann-Whiteney test. J: Heat map of flow speed showing detailed regions within the KV in control and crispant embryos at the 8 ss. The pseudo-color scale represents flow speed in ?m/s, where white represents high speed versus low speed in blue. K: Box plots for instantaneous flow speed measured at different locations of the KVs, based on the same data set used to generate the heat maps. Box plots display the median with a vertical line, and the whiskers represent the minimum and maximum values observed. Control vs. crispants, Ne, number of embryos; ntracks, number of particle tracks followed. Ne = 2/2, overall ntracks = 599/168, anterior ntracks = 235/65, posterior ntracks = 364/103, left ntracks = 358/86, right ntracks = 241/82. ????, P < 0.0001; Mann-Whiteney test. Scale bars, 30 ?m (C); 20 ?m (E). L, left; R, right; KV, Kupffer?s vesicle.