FIGURE

Figure 4

ID

ZDB-FIG-240902-21

Publication

Abate et al., 2024 - The Bioactive Gamma-Oryzanol from Oryza sativa L. Promotes Neuronal Differentiation in Different In Vitro and In Vivo Models

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Figure 4

Gamma-oryzanol positively affects neuronal differentiation of ahNPCs. AhNPCs are seeded in differentiating conditions in the presence of a vehicle (DMSO 0.2%) or gamma-oryzanol (ORY) at 1, 5, or 10 μg/mL for 24 h. (A) Percentage of MAP2+ cells over total viable cells. (B) Percentage of GFAP+ cells over total viable cells. (C) Quantification of apoptotic cells calculated over the total number of Hoechst+ nuclei. (D–G) Representative confocal microscope images of MAP2+ (red, (D,E)) and GFAP+ (green, (F,G)) cells derived from ahNPCs differentiated in the presence of a vehicle (veh, (D,F)) or ORY (10 μg/mL, (E,G)). Nuclei are stained with Hoechst (blue). Magnification 63X, Scale bar = 20 µm. Data are represented as means ± SD of n = 3 experiments run in triplicate. Student’s t-test. *** p < 0.001; ** p < 0.01; * p < 0.05 vs. DMSO-treated cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)