Figure 5

Gamma-oryzanol positively regulates nervous system development in zebrafish. (A–F) Representative images of the head region of 2 dpf Tg (-3.1neurog1:GFP) zebrafish embryos injected with std–MO or nrf2–MOs and then with DMSO or ORY: (A) std–MO + DMSO (n = 11); (B) nrf2a–MO + DMSO (n = 10); (C) nrf2b–MO + DMSO (n = 10); (D) std–MO + ORY (n = 18); (E) nrf2a–MO + ORY(n = 11); (F) nrf2b–MO + ORY (n = 10). (G) Quantification of the GFP fluorescence intensity of std–MO or nrf2–MOs embryos injected with DMSO or ORY. (H–M) islet1 whole mount in situ hybridization of std–MO or nrf2–MOs embryos injected with DMSO or ORY: (H) std–MO + DMSO (n = 17); (I) nrf2a–MO + DMSO(n = 15); (J) nrf2b–MO + DMSO (n = 14); (K) std–MO + ORY (n = 15); (L) nrf2a–MO + ORY (n = 18); (M) nrf2b–MO + ORY (n = 11). (N) Subdivision of std–MO or nrf2–MOs embryos injected with DMSO or ORY according to the relative islet1 expression. (O,P) RT-qPCR expression analyses of (O) gstp1 and (P) bdnf. Scale bar indicates 100 μm. Data are presented as means ± standard error of the mean (S.E.M.). One–Way ANOVA with Tukey post–hoc. *** p < 0.001; ** p < 0.01; * p < 0.05; ns: not significant.