Figure 4

Figure 4

Gamma-oryzanol positively affects neuronal differentiation of ahNPCs. AhNPCs are seeded in differentiating conditions in the presence of a vehicle (DMSO 0.2%) or gamma-oryzanol (ORY) at 1, 5, or 10 μg/mL for 24 h. (A) Percentage of MAP2+ cells over total viable cells. (B) Percentage of GFAP+ cells over total viable cells. (C) Quantification of apoptotic cells calculated over the total number of Hoechst+ nuclei. (D–G) Representative confocal microscope images of MAP2+ (red, (D,E)) and GFAP+ (green, (F,G)) cells derived from ahNPCs differentiated in the presence of a vehicle (veh, (D,F)) or ORY (10 μg/mL, (E,G)). Nuclei are stained with Hoechst (blue). Magnification 63X, Scale bar = 20 µm. Data are represented as means ± SD of n = 3 experiments run in triplicate. Student’s t-test. *** p < 0.001; ** p < 0.01; * p < 0.05 vs. DMSO-treated cells.