FIGURE

Fig. 6

ID

ZDB-FIG-240814-50

Publication

Farmer et al., 2024 - Cellular transitions during cranial suture establishment in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Mid-suture mesenchyme upregulates grem1a and does not contribute to bone. A Confocal images show Alizarin Red staining of frontal (F) and parietal (P) bones relative to induction of grem1a:nlsEOS in forming sutures (arrowheads). Asterisks denote brain expression. N = 5 each stage. B Schematic of conversion scheme for calvaria. grem1a+ cells were globally converted, imaged, and re-imaged at 28 days post conversion (dpc). C Confocal images of grem1a:nlsEOS+ calvaria at 0 and 28 dpc. N = 6. D Orthogonal section of adult coronal suture shows grem1a:nlsEOS in mid-suture cells non-overlapping with sp7:mCherry+ osteoblasts. N = 8. E Quantification of grem1a:nlsEOS single-positive and grem1a:nlsEOS; sp7:mCherry double-positive cells at cranial sutures. Scalebars: 200 μM (A, B), 50 μM (D). P-values were calculated using a two-tailed non-parametric Student’s t tests. Error bars represent S.E.M. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.