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Fig. 6 Mid-suture mesenchyme upregulates grem1a and does not contribute to bone. A Confocal images show Alizarin Red staining of frontal (F) and parietal (P) bones relative to induction of grem1a:nlsEOS in forming sutures (arrowheads). Asterisks denote brain expression. N = 5 each stage. B Schematic of conversion scheme for calvaria. grem1a+ cells were globally converted, imaged, and re-imaged at 28 days post conversion (dpc). C Confocal images of grem1a:nlsEOS+ calvaria at 0 and 28 dpc. N = 6. D Orthogonal section of adult coronal suture shows grem1a:nlsEOS in mid-suture cells non-overlapping with sp7:mCherry+ osteoblasts. N = 8. E Quantification of grem1a:nlsEOS single-positive and grem1a:nlsEOS; sp7:mCherry double-positive cells at cranial sutures. Scalebars: 200 μM (A, B), 50 μM (D). P-values were calculated using a two-tailed non-parametric Student’s t tests. Error bars represent S.E.M. Source data are provided as a Source Data file.