PUBLICATION
Cellular transitions during cranial suture establishment in zebrafish
- Authors
- Farmer, D.T., Dukov, J.E., Chen, H.J., Arata, C., Hernandez-Trejo, J., Xu, P., Teng, C.S., Maxson, R.E., Crump, J.G.
- ID
- ZDB-PUB-240814-3
- Date
- 2024
- Source
- Nature communications 15: 69486948 (Journal)
- Registered Authors
- Crump, Gage DeKoeyer
- Keywords
- none
- Datasets
- GEO:GSE223147
- MeSH Terms
-
- Signal Transduction
- Single-Cell Analysis
- Osteogenesis*
- Gene Expression Regulation, Developmental
- Animals
- Mesoderm*/cytology
- Mesoderm*/embryology
- Mesoderm*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Bone Morphogenetic Proteins*/genetics
- Bone Morphogenetic Proteins*/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Skull/embryology
- Cranial Sutures*/embryology
- Cranial Sutures*/growth & development
- Cranial Sutures*/metabolism
- Mutation
- PubMed
- 39138165 Full text @ Nat. Commun.
Citation
Farmer, D.T., Dukov, J.E., Chen, H.J., Arata, C., Hernandez-Trejo, J., Xu, P., Teng, C.S., Maxson, R.E., Crump, J.G. (2024) Cellular transitions during cranial suture establishment in zebrafish. Nature communications. 15:69486948.
Abstract
Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping