FIGURE

Fig. 6

ID

ZDB-FIG-240725-34

Publication

Li et al., 2024 - Biosynthetic deficiency of docosahexaenoic acid causes nonalcoholic fatty liver disease and ferroptosis-mediated hepatocyte injury

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Fig. 6

NAFLD in elovl2 mutant were rescued by fat1-transgenesis. A, fatty acid composition in ?/? and ?/?, fat1 livers. B, HE (hematoxylin-eosin) staining of ?/? and ?/?, fat1 liver sections. Scale bar: 25 ?m. C and D, Oil-Red staining of ?/? and ?/?, fat1 liver sections, and quantitative analysis of Oil-Red staining signals. Scale bar: 50 ?m. E, qRT-PCR analysis of genes involved in lipogenesis in ?/? and ?/?, fat1 livers. N = 3 replicates. F, analysis of mitochondria ultrastructure in the hepatocytes of ?/? and ?/?, fat1 liver sections. m: mitochondria. Scale bar: 500 nm. G, analysis of ATP production in ?/? and ?/?, fat1 livers. H, analysis of ER (endoplasmic reticulum) ultrastructure in the hepatocytes of ?/? and ?/?, fat1 liver sections. er: white arrow indicated. scale bar: 500 nm. I, qRT-PCR analysis of genes involved in ER stress in ?/? and ?/?, fat1 livers. N = 3 replicates. J, qRT-PCR analysis of genes involved in inflammation in the liver of ?/? and ?/?, fat1 livers. N = 3 replicates. K and L, TUNEL (TdT-mediated dUTP Nick-End Labeling) assays in ?/? and ?/?, fat1 liver sections. Scale bar: 50 ?m. M, qRT-PCR analysis of gpx4a, gpx4b, acsl4a, acsl4b in the liver of ?/? and ?/?, fat1 livers. N = 3 replicates. All values are mean � SD. A Student t test was used. ?p < 0.05, ??p < 0.01, ???p < 0.001. Individual p values are listed in Table S8 . ?/?, elovl2?/?; fat1, elovl2?/?/fat1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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