Sibling larvae at 7 dpf do show distinct habituation response post 5 min rest, but vps16(-/-) mutants do not show distinct response. (A) Schematic representation of intermediate memory paradigm showing round one stimulus having 10 high-intensity taps, followed by 5 min rest period with no stimulation, and finally round two stimulus having 10 high-intensity taps. (B) Line graph representing average distance traveled in response to each individual tap by 7 dpf siblings (blue, n = 60) and vps16(-/-) mutants (green, n = 59) larvae before and after 5 min rest period (red). Box plots representing median distance traveled by 7 dpf siblings (blue, n = 60) and vps16(-/-) (green, n = 59) larvae in response to multiple-tap stimuli before and after 5 min rest period (red). First-order exponential decay curve representing average distance traveled in response to round one multiple-tap stimuli by 7 dpf siblings (blue, n = 60) and vps16(-/-) mutant (green, n = 59) larvae before and after 5 min rest period (red). For all graphs, “N.S.” = not significant, “****” = p < 0.0001, and error bars indicate SEM.
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