FIGURE

Fig. 4

ID

ZDB-FIG-240708-43

Publication

McKaige et al., 2024 - Mitochondrial abnormalities contribute to muscle weakness in a Dnajb6 deficient zebrafish model

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Fig. 4

Analysis of autophagy markers reveals early defect in double mutants. (A) Double mutant fish show a significant reduction in autophagic flux activity at 6 dpf based on difference in LC3-II levels between NH4Cl treated and untreated zebrafish. Experiment was completed in triplicate. (B) Western blot at 6 dpf shows a significant accumulation of p62 in double mutants. Error bars represent SEM for three biological replicates. (C) At 3 mpf dnajb6b single mutants and double mutants show a significant reduction in p62 levels compared to wildtype. Error bars represent SEM for eight fish across 3 biological replicates. GAPDH was used as a loading control and P62 values are normalised to GAPDH. Datasets were analysed with a one-way ANOVA and a two-way Dunnett multiple comparisons test.

Expression Data

Antibodies:	Ab6-sqstm1 Ab11-map1lc3
Fish:	TU dnajb6a^mnu13/mnu13 (TU) dnajb6b^mnu14/mnu14 (TU) dnajb6a^mnu13/mnu13; dnajb6b^mnu14/mnu14 (TU)
Condition:	chemical treatment by environment: ammonium chloride
Anatomical Terms:	muscle whole organism
Stage Range:	Day 6 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU dnajb6a^mnu13/mnu13 (TU) dnajb6b^mnu14/mnu14 (TU) dnajb6a^mnu13/mnu13; dnajb6b^mnu14/mnu14 (TU)
Condition:	chemical treatment by environment: ammonium chloride
Observed In:	muscle whole organism
Stage Range:	Day 6 to Adult

Phenotype Detail

Acknowledgments

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