FIGURE

Fig. 3

ID

ZDB-FIG-240621-13

Publication

Lalonde et al., 2024 - pIGLET: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish

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Fig. 3

loxP Switch transgenes have predictable and consistent activity in pIGLET14a and pIGLET24b. Comparison of CreERT2-mediated recombination of pIGLET-based loxP reporter hsp70l:Switch2. Transgene and crossing schematics on top, lateral confocal views and anterior to the left. (A and B) Testing of p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 with three independent CreERT2-driver lines: ubi:creERT2, drl:creERT2, and crestin:creERT2. p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 drive strong mApple reporter expression following CreERT2-mediated recombination when crossed to ubiquitous and tissue-specific CreERT2-driver lines, treated with 4-OHT, and imaged at 56 hpf (A and B). Note the near-complete mApple labeling of the embryo at 56 hpf when p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 are combined with ubi:creERT2 and accurate labeling of the LPM or neural crest cells when used in combination with drl:creERT2 or crestin:creERT2, respectively, akin to previous results using Tol2-based versions of either CreERT2 driver (A and B). F2 heterozygous embryos are depicted (A and B). Scale bar, 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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