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Fig. 3 loxP Switch transgenes have predictable and consistent activity in pIGLET14a and pIGLET24b. Comparison of CreERT2-mediated recombination of pIGLET-based loxP reporter hsp70l:Switch2. Transgene and crossing schematics on top, lateral confocal views and anterior to the left. (A and B) Testing of p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 with three independent CreERT2-driver lines: ubi:creERT2, drl:creERT2, and crestin:creERT2. p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 drive strong mApple reporter expression following CreERT2-mediated recombination when crossed to ubiquitous and tissue-specific CreERT2-driver lines, treated with 4-OHT, and imaged at 56 hpf (A and B). Note the near-complete mApple labeling of the embryo at 56 hpf when p14a.hsp70l:Switch2 and p24b.hsp70l:Switch2 are combined with ubi:creERT2 and accurate labeling of the LPM or neural crest cells when used in combination with drl:creERT2 or crestin:creERT2, respectively, akin to previous results using Tol2-based versions of either CreERT2 driver (A and B). F2 heterozygous embryos are depicted (A and B). Scale bar, 200 μm.