FIGURE

Fig. 4

ID

ZDB-FIG-240619-19

Publication

Zhang et al., 2024 - TAF1 is needed for the proliferation and maturation of thyroid follicle cells via Notch signaling

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Fig. 4

Notch signaling was impaired after TATA-box binding protein associated Factor 1 (TAF1) knockdown. Human Nthy-ori 3-1 cells were stably transfected with either control shRNA (Vector) or TAF1-specific shRNA (TAF1 shRNA). A: EdU proliferation assays were performed to determine the proliferative ability (green fluorescence: EdU, blue fluorescence: DAPI). B: the ratio of EdU-positive cells to DAPI-positive cells. C: the results of cell viability analysis verification by CCK8. A: the results of CCK8. D: the expression of TAF1, Notch intracellular domain (NICD), HES1, SLC5A5, and ACTIN was determined by Western blot (n = 3). E: the relative protein expression (protein/ACTIN) was quantified by ImageJ. Scale bars: 100 μm. **P < 0.01, ***P < 0.001.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Endocrinol. Metab.