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Fig. 4 Notch signaling was impaired after TATA-box binding protein associated Factor 1 (TAF1) knockdown. Human Nthy-ori 3-1 cells were stably transfected with either control shRNA (Vector) or TAF1-specific shRNA (TAF1 shRNA). A: EdU proliferation assays were performed to determine the proliferative ability (green fluorescence: EdU, blue fluorescence: DAPI). B: the ratio of EdU-positive cells to DAPI-positive cells. C: the results of cell viability analysis verification by CCK8. A: the results of CCK8. D: the expression of TAF1, Notch intracellular domain (NICD), HES1, SLC5A5, and ACTIN was determined by Western blot (n = 3). E: the relative protein expression (protein/ACTIN) was quantified by ImageJ. Scale bars: 100 μm. **P < 0.01, ***P < 0.001.