Fig. 4.

Microtubules and the microtubule minus end-directed motor protein dynein drive NE-induced color change. (A) JF549-labeled iridophores (Left) and corresponding kymographs of untreated (Top), nocodazole-treated (Middle), and NE-treated cells (Bottom). Kymograph Insets are contrasted for clarity. (B) Microtubule distribution visualized using an anti-α-tubulin antibody in an iridophore (arrow) and an adjacent dermal cell (arrowhead). (C) Iridophore in a scale after 10 min of recovery from nocodazole treatment (anti-α-tubulin, cyan; crystals autofluorescence, magenta). (D) TEM image of an iridophore showing ~25 nm tubes running parallel to the crystals. (E–H) Graphs showing the blue-to-red channel ratio (the deconvoluted components of an RGB movie) of iridophores treated with NE (E), nocodazole + NE (F), dynapyrazole-a + NE (G) and adenosine (H). (Scale bars, (A, Left) 4 µm, (A, Right) 10 µm, (B) 8 µm, (C) 5 µm, (D) 400 nm.)