FIGURE

Fig. 3

ID

ZDB-FIG-240603-89

Publication

Miyazaki et al., 2024 - DHCR7 links cholesterol synthesis with neuronal development and axonal integrity

Other Figures

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Fig. 3

Impaired myelination and autophagy in dhcr7?/? zebrafish (A) Representative Kl�ver?Barrera staining of transverse sections of 3-mpf dhcr7+/+ and dhcr7?/? zebrafish telencephalon and optic tectum. Arrows indicate myelin stained in the dhcr7+/+ telencephalon. (B), (C) Representative transmission electron micrographs of the telencephalon (B) and optic tectum (C) from 2-mpf dhcr7+/+ and dhcr7?/? zebrafish. Arrows indicate axonal lysosome-like structures; arrowheads indicate mitochondria with indistinct cristae, which appeared expanded. (D) Lysosome staining by Lysoprime Green in 2-mpf dhcr7+/+ and dhcr7?/? zebrafish telencephalon (n = 5 each). (E) Western blot analysis of p62 and Lc3b protein levels in 2-mpf dhcr7+/+ and dhcr7?/? zebrafish brains (n = 5 each) relative to GAPDH. All values are presented as the mean � SEM. *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Antibodies:	Ab1-map1lc3b Ab4-sqstm1
Fish:	WT dhcr7^oi14/oi14
Anatomical Term:	brain
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dhcr7^oi14/oi14
Observed In:	brain optic tectum myelination telencephalon lysosome telencephalon myelination
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.