FIGURE

Fig. 2

ID

ZDB-FIG-240603-32

Publication

Wu et al., 2019 - Bax inhibitor 1 is a γ-secretase-independent presenilin-binding protein

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Fig. 2

BI1 and PS1 form a stable complex. (A) Subcellular fractionation shows colocalization of FLAG-tagged PS1 and His6-tagged BI1. The PNS of HEK293F cells was fractionated on 30% Percoll and subjected to Western blot analysis using a monoclonal antibody against the His6 or FLAG tag. (B) PS1 and BI1 colocalize in HEK293 cells. PS1 and BI1 were fused with GFP and RFP, respectively, at their N or C termini. In all four combinations, PS1 and BI1 colocalize in cells by confocal microscopy. (Scale bar, 10 μm.) (C) PS1 interacts with BI1 in the IP-Western analysis. The cellular extract was immunoprecipitated using an anti-FLAG monoclonal antibody, and the pellet was examined by Western blot (WB) using anti-FLAG and anti-His6 antibodies. IP, immunoprecipitation. (D) PS1 and BI1 form a stable complex. The recombinant FLAG-tagged PS1 and His6-tagged BI1 were individually or together applied to gel filtration. The fractions were examined by Western blot.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA