FIGURE

Fig. 2

ID

ZDB-FIG-240603-32

Publication

Wu et al., 2019 - Bax inhibitor 1 is a γ-secretase-independent presenilin-binding protein

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Fig. 2

BI1 and PS1 form a stable complex. (A) Subcellular fractionation shows colocalization of FLAG-tagged PS1 and His6-tagged BI1. The PNS of HEK293F cells was fractionated on 30% Percoll and subjected to Western blot analysis using a monoclonal antibody against the His6 or FLAG tag. (B) PS1 and BI1 colocalize in HEK293 cells. PS1 and BI1 were fused with GFP and RFP, respectively, at their N or C termini. In all four combinations, PS1 and BI1 colocalize in cells by confocal microscopy. (Scale bar, 10 ?m.) (C) PS1 interacts with BI1 in the IP-Western analysis. The cellular extract was immunoprecipitated using an anti-FLAG monoclonal antibody, and the pellet was examined by Western blot (WB) using anti-FLAG and anti-His6 antibodies. IP, immunoprecipitation. (D) PS1 and BI1 form a stable complex. The recombinant FLAG-tagged PS1 and His6-tagged BI1 were individually or together applied to gel filtration. The fractions were examined by Western blot.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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