Fig 1

Zebrafish mylipb is upregulated upon poly I:C treatment and SVCV infection.

(A) Zebrafish mylipb as well as ifn1, but not mylipa mRNA was induced by poly I:C in ZFL cells. ZFL cells were seeded in 60-mm plates overnight and treatment with poly I:C (1 μg/mL) for indicate time. (B) Zebrafish mylipb as well as ifn1, but not mylipa mRNA was induced by SVCV (virus strain OMG067) infection (MOI of 1) in ZFL cells. ZFL cells were seeded in 60-mm plates overnight and infected with SVCV for different time. (C) Zebrafish mylipa but not mylipb mRNA was induced by LXR agonist T0901317 in a dose-dependent manne in ZFL cells. ZFL cells were seeded in 60-mm plates overnight and treatment with T0901317 for 24 h. (D) Zebrafish mylipa but not mylipb mRNA was induced by LXR agonist T0901317 (1 μM) in a indicate time in ZFL cells. ZFL cells were seeded in 60-mm plates overnight and treatment with T0901317 for indicate time. Total RNA was extracted for examining the expression of target genes by quantitative real-time PCR (qRT-PCR). All data are presented as mean values based on three repeated experiments, and error bars indicate the ± SD. *,P < 0.05, **, P<0.01; ***, P < 0.001; ****, P< 0.0001.