ap2s1 regulates behavioral performance and choice in goal-directed and spontaneous contexts (A) OMR score of siblings (blue, n = 16) and ap2s1p172 mutants (red, n = 11) to moving optomotor visual stimuli, where positive scores indicate overall movement in the same direction as the visual stimuli. ∗p < 0.05, two-tailed Mann-Whitney test. (B) Relative fish movement trajectories following the onset of each leftward (green) or rightward (orange) optomotor stimulus, depicted starting from the black cross at the center of each graph. Presented paths of siblings (left graph) and mutants (right graph) were truncated upon exiting a square region of interest during the trial (see STAR Methods). (C) Relative consumption of rotifers by non-mutant siblings (blue, n = 23), ap2s1p172 mutants (red, n = 11), and control arenas with no fish (black, n = 3) over a 135-min time period (∗∗∗p ≤ 0.001, two-tailed t-test with Welch’s correction, at conclusion of assay). Rotifer counts for each individual were normalized to 100% at time 0. (D and E) Sibling and ap2s1p172 mutant spontaneous behavior in the light (n = 52 siblings, n = 33 mutants) and the dark (n = 72 siblings, n = 18 mutants). ∗∗∗∗p < 0.0001, two-tailed Mann-Whitney test. See also Figure S2 for details. Data are presented as mean ± SEM in all panels.
|
