Fig. 6

Genetic and chemical depletion of macrophages in zebrafish embryos.

Primitive macrophages were genetically and chemically depleted before cell engraftment in Tg (mpeg1:mCherry) zebrafish embryos. A–D Complete and efficient depletion of macrophages is observed in PU.1 morphant (B) and in Lipo-C-treated (D) embryos at 35 h.p.f and 48 h.p.f, respectively, compared to controls (A and C) which contain fluorescent macrophages (head arrows). E JK-GFP cells injected in PU.1 treated and non-treated zebrafish embryos are followed for 6 days. Injection into the Duct of Cuvier (DC) of MO-buffer-treated embryos. Injection into the Duct of Cuvier (DC) of PU.1 Morphant embryos. F, G Transplantations of JK-GFP (F) and CD34-GFP (G) cells in the Duct of Cuvier (DC) of Lipo-C-treated embryos show a percentage of engraftment comparable to that obtained with injections into the yolk sac. Scale bar: 250 µm. Injection in the yolk sac of PBS-treated embryos. Injection into the Duct of Cuvier (DC) of PBS-treated embryos. Injection into the Duct of Cuvier (DC) of Lipo-C-treated embryos. Data were presented as mean ± SD and are representative of 3 independent experiments with n = 15–20 (E), n = 10–30 (F) and n = 10–30 (G) embryos/group. (ns not significant or p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).