FIGURE

Fig. 3

ID

ZDB-FIG-240505-3

Publication

El Omar et al., 2024 - Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Human JK-GFP cells phagocytosis by zebrafish embryonic macrophages.

A Live imaging of JK-GFP cells injected in a zebrafish embryo at 30 h.p.f. (arrows indicate two cells). Starting from 33 min post transplantation, some green cells appear fragmented (C, D arrows). D Inset shows a high magnification of fragmented cells at 61 min (arrows). Each frame is a maximum projection of 3 planes apart 1 μm. See also Video 4. E–J Macrophages recruitment in a 4-d.p.f. Tg (mpeg1:mCherry) zebrafish embryo after injection with PBS (E–G) or with JK-GFP cells (H–J) (arrow). Merged picture (J) shows the accumulation of macrophages around JK-GFP cells. K Live imaging of mCherry-macrophages in a 4-d.p.f. embryo, 2 days after transplantation of JK-GFP cells. High magnification reveals the phagocytosis of green human cells engulfed by red host macrophages. Each frame is a maximum projection of 5 planes apart 0.8 μm.See also Video 5. Scale bars: (A–D): 25 µm, (E–J): 250 µm, (K): 20 µm. Time code in minutes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.