FIGURE

Fig. 6

ID

ZDB-FIG-240425-6

Publication

Rayner et al., 2024 - Cyclin F can alter the turnover of TDP-43

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Fig. 6

Mutant cyclin F promotes TDP-43 degradation through the proteasome. A. HEK293T cells were triple transfected with TDP-43-GFP, His-ubiquitin and mCherry-cyclin F or empty vector control. Cells were treated with either 10 μM MG132 or 10 μM Chloroquine (CQ) for 4 h before lysis. Lysates were analysed by immunoblotting using the antibodies indicated. B. His-ubiquitin pull-downs (His-UB pulldown) from lysates (shown in A) were conducted under denaturing conditions. Eluents were analysed by immunoblotting using an antibody recognising TDP-43. C. Densitometry analysis of TDP-43 bands results from B. N = 3. Results represent the mean and SD. ***p ≤ 0.001, ns; not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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