Fig. 7

Cyclin FMRL/AAA does not increase developmental abnormalities in zebrafish. A. Representative images of zebrafish at 24 h post fertilisation demonstrating typical morphology of embryos classified as normal, mild and severe abnormalities. Scale bar = 500 μm. B. Quantification of normal embryo morphology at 24 hpf following injection with mCherry, mCherry-cyclin FWT or mCherry-cyclin FMRL/AAA mRNA. C. Quantification of embryos with mild abnormalities at 24 hpf. D. Quantification of embryos with severe abnormalities at 24 hpf. E. Quantification of non-viable embryos at 24 hpf. F. Equal expression of cyclin F in mCherry-cyclin FWT and mCherry-cyclin FMRL/AAA groups was confirmed by western blot followed by densitometry using ImageJ, confirming differences observed between groups were not a result of differing levels of overexpression. All graphs represent mean with SD of three independent experiments. An unpaired t-test was used to determine statistical significance. *p ≤ 0.05, **p ≤ 0.01, ns; not significant. The western blot has been spliced as indicated by the dotted lines.