|
Fig. 6 Mutant cyclin F promotes TDP-43 degradation through the proteasome. A. HEK293T cells were triple transfected with TDP-43-GFP, His-ubiquitin and mCherry-cyclin F or empty vector control. Cells were treated with either 10 μM MG132 or 10 μM Chloroquine (CQ) for 4 h before lysis. Lysates were analysed by immunoblotting using the antibodies indicated. B. His-ubiquitin pull-downs (His-UB pulldown) from lysates (shown in A) were conducted under denaturing conditions. Eluents were analysed by immunoblotting using an antibody recognising TDP-43. C. Densitometry analysis of TDP-43 bands results from B. N = 3. Results represent the mean and SD. ***p ≤ 0.001, ns; not significant.