Fig. 4

IL-16 deficiency promoted phagosome conversion in mycobacterium-infected macrophages. A to B WT and Il-16-/- BMDMs were infected with M.marinum (MOI = 10:1) in the presence or absence of rmL-16 protein (10 ng/mL). On day 2 post-infection, Rabbit Abs for M. marinum, mouse Abs for Lamp-1 (A), and mouse Abs for cathepsin D (B) were used. C Percent of co-localization of M. marinum and Lamp-1 or cathepsin D, according to A and B. D WT and Il-16-/- BMDMs were infected with H37Rv (MOI = 3:1) in the presence or absence of rmL-16 protein (10 ng/ml). On day 2 post-infection, Rabbit Abs for H37Rv, mouse Abs for Lamp-1, and mouse Abs for cathepsin D were used. E Percent of co-localization of H37Rv and Lamp-1 or cathepsin D, according to D. F Il-16-/- BMDMs were infected with H37Rv (MOI = 3:1) in the presence or absence of rmL-16 protein (10 ng/mL), rabbit anti CD4 or IL-16 and control IgG were added. On day 2 post-infection, Rabbit Abs for H37Rv, mouse Abs for Lamp-1, and mouse Abs for cathepsin D were used. G Western blotting analysis was used to examine the activation of PI3 K, AKT, mTOR, according to F. *p<0.05 compared to media, Student's t-test. The graph shown is representative of 2 independent experiments.