Fig. 3

IL-16 secretion controls mycobacterium intracellular survival. A Alveolar macrophages (AMs) from wild-type (WT) or Il-16-/- mice were infected H37Ra at an MOI of 5:1 for 4 h. The mycobacterium uptake was measured by CFU counting at 1 hpi (n = 7 to 8). B Human monocytes-derived macrophages were pretreated with or without neutralized antibody anti-IL-16 (1 µg/mL), rhL-16 protein (10 ng/mL) for 18 h, infected with H37Rv (MOI = 3:1) for 4 h. Mycobacterium load was measured by CFU counting at 1, 3, and 7 dpi (n = 4 to 7). C H37Ra load was measured by CFU counting at 1 and 5 dpi, according to (A) (n = 6 to 8). D Bone Marrow-Derived Macrophages (BMDMs) from WT or Il-16-/- mice were infected H37Rv at an MOI of 3:1 for 4 h. The mycobacterium load was measured by CFU counting at 0 to 9 dpi (n = 3). *p<0.05 compared to media, Student's t-test. The graph shown is representative of 2 independent experiments.