FIGURE 3

Plexin B3 synergizes genetically with Neuropilin1 and Sema3E in retinal axon guidance. Half doses of Plexin B3, Nrp1a or Sema3E MO results in low proportion of ipsilateral misprojections of retinal axons. Combining half doses of Plexin B3 MO with Nrp1a or Sema3E MO causes dramatic increase of ipsilateral misprojections, which is much more than the simple sum up of the individual half doses of MOs. The combo results strongly indicate that Plexin B3 genetically interact with Nrp1a and Sema3E and might function as receptor-ligand in axon guidance in vivo. Stars above the columns indicate that the proportion of eyes with ipsilateral misprojections induced by the combination of half doses is significantly higher than would be expected by assuming that the effects of half doses add together independently. ***p < 0.001, Fisher’s exact test.