FIGURE

Figure 4.

ID

ZDB-FIG-240416-45

Publication

Li et al., 2024 - FARS2 Deficiency Causes Cardiomyopathy by Disrupting Mitochondrial Homeostasis and the Mitochondrial Quality Control System

Other Figures

All Figure Page

Back to All Figure Page

Figure 4.

Defective mitochondrial homeostasis and protein synthesis in icKO hearts. A, Gene Ontology analysis of mitochondrial homeostasis–related biology process changes. B, Heatmap of mitochondrial aminoacyl-tRNA synthetase (mtARS) genes of inducible cardiac-specific Fars2 knockout (icKO) compared with wild-type (WT) mice from RNA sequencing analysis. C, Top, aminoacylation assay for mitochondrial mt-tRNA^Phe in mouse heart. Charged (Phe-mt-tRNA^Phe) and uncharged tRNA (mt-tRNA^Phe) are indicated. Bottom, quantification of Phe-mt-tRNA^Phe/mt-tRNA^Phe from the top panel (n=3). D, Western blots of oxidative phosphorylation (OXPHOS) complexes and Fars2 in WT and icKO mice. E, Quantification of the OXPHOS complexes from D (n=3). F and G, Expression levels of mitochondrial DNA (mtDNA) coding genes in the heart of icKO mice at 3 weeks (F) and icKO mice at 10 weeks (G; n=3 mice per group). *P<0.05; **P<0.01; ***P<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Circulation