FIGURE

Fig. 3.

ID

ZDB-FIG-240408-68

Publication

Dorner et al., 2024 - Efficient genome editing using modified Cas9 proteins in zebrafish

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Fig. 3.

Improvement of knockout and repair efficiencies by adding an aNLS. In A the efficiencies of knocking out the function of the albino gene are compared between Cas9 and aNLS-Cas9 and SpRY and aNLS-SpRY. Whereas in the case of Cas9 the addition of an aNLS leads to a significantly higher efficiency, 93.5% in the best two categories compared to 55.1%, this is not the case for SpRY. Detailed results see Table S3. Purified aNLS-Cas9 performs as well as commercially available Cas9 protein (Alt-R™ S.p. Cas9 V3, IDT) when tested for the knockout of the albino gene (B). In addition, the repair efficiency for aNLS-SpRY is significantly higher compared to SpRY, with 19% versus 9.1% in the category ‘good’ (C). For detailed results see Tables S4 and S5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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