Fig. 1.

Efficient knockout of the albino gene using the SpRY protein. In A the albino locus is schematically shown, exons are in grey, introns are shown as gaps, not to scale. The coding sequence is in dark grey and the different target sites in exons 1, 3 and 6 are indicated, the position of the alb^b4 mutation in exon 6 is marked by a red asterisk. The different categories for the evaluation of the knockout efficiency are shown in B-F′. Lateral views (B-F) and dorsal views (B′-F′) of larvae 3 dpf are shown, scale bar: 2 mm. Category 1, no knockout (none) (B), (B′), category 2 weak (C), (C′), category 3 moderate (D), (D′), category 4 good (E), (E′) and category 5 very good/complete knockout (F), (F′). In G the results for the six target sites tested are shown. For detailed results see Table S1.