Fig. 2.

Homology directed repair of the albino^b4 mutation using the Cas9 variant SpRY. In A the partial sequence of exon 6 of the albino gene is depicted, coding sequence in capital letters, intron sequence in small letters. The target sites U3, U4, U5 and U6* are indicated by arrows; the mutation in alb^b4 leading to a premature stop codon is shown in red and marked with an asterisk. In B the oligonucleotides used as donors for HDR are shown. The two base pairs that are altered are shown in red; note the 5′ and 3′ ends of the oligonucleotides indicating the DNA strand they correspond to. The different categories for the evaluation of the repair efficiency are shown in C-F′. Lateral views (C-F) and dorsal views (C′-F′) of larvae 3 dpf are shown, scale bar: 2 mm. Category 1, no repair (none) (C), (C′), category 2 weak (D), (D′), category 3 moderate (E), (E′) and category 4 good repair (F), (F′). In G the results for the six tested donor oligonucleotides are shown. For detailed results see Table S2.