FIGURE

Figure 6

ID

ZDB-FIG-240405-88

Publication

Diana et al., 2024 - Differentiation and functioning of the lateral line organ in zebrafish require Smpx activity

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Figure 6

Smpx is required for normal kinocilium structure. Immunofluorescence performed on whole mount 120 hpf larvae with an antibody against acetylated tubulin to label the neuromast kinocilia. (A,B) Compared to controls (ctrlMO), the kinocilia of morphant larvae (smpxMO) appear clearly shorter in length and numerically less dense. (C,D) As for the knockdown experiment, when compared to controls (ctrl), the kinocilia of crispant larvae (smpx^crispant) are shorter and numerically lower. (A,B) ctrlMO n = 5, smpxMO n = 4. (C,D) ctrlMO n = 5, smpxMO n = 5. (A–D) Confocal Z-stack images of neuromast L4 for each larva. n is equal to the sum of the larvae from two independent experiments. Embryos were counterstained with DAPI to visualize cell nuclei. Scale bars are 6 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR5-smpx + CRISPR6-smpx + CRISPR7-smpx WT + MO1-smpx
Knockdown Reagents:	CRISPR5-smpx CRISPR6-smpx CRISPR7-smpx MO1-smpx
Observed In:	neuromast hair cell kinocilium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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