FIGURE

Figure 4

ID

ZDB-FIG-240325-13

Publication

Lv et al., 2024 - The splicing factor Prpf31 is required for hematopoietic stem and progenitor cell expansion during zebrafish embryogenesis

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Figure 4

HSPC proliferation in the CHT of prpf31^−/−zebrafish is arrested in M phase.A, double staining of cmyb:EGFP and EdU showed no obvious difference of EdU⁺ HSPCs in the AGM and CHT at 36 and 48 hpf. The total number of embryos examined were indicated at the top right of each panel. The scale bars represent 50 μm. B, quantification of the percentage and number of EdU⁺ HSPCs was detected in (A). The total number of embryos examined was indicated below each column. Mean ± SD; unpaired two-tailed t test; ∗p < 0.05, ∗∗p < 0.01; ns, not significant. C, double immunostaining of cmyb:EGFP and pH3 (Ser10) showed a significant increase of pH3⁺ HSPCs in the CHT at 48 hpf, but not in the AGM and CHT at 36 hpf. The total number of embryos examined were indicated at the top right of each panel. The scale bars represent 50 μm. D, quantification of the percentage and number of pH3⁺ HSPCs was detected in (C). The total number of embryos examined was indicated below each column. Mean ± SD; unpaired two-tailed t test; ∗p < 0.05, ∗∗∗p < 0.001; ns, not significant. AGM, aorta-gonad-mesonephros; CHT, caudal hematopoietic tissue; EdU, 5-ethynyl-29-deoxyuridine; hpf, hours postfertilization; HSPC, hematopoietic stem and progenitor cell; pH3, phospho-histone 3. EGFP, enhanced green fluorescent protein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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