Expression patterns of prpf31 during zebrafish embryonic development in siblings and prpf31−/−zebrafish.A–L and A’–L’, prpf31 expression in siblings and prpf31−/− zebrafish was examined by WISH at different developmental stages. E–L and E’–L’: lateral views, anterior to the left and dorsal upward. G and G’: magnified views of the ICM in (E and E’). H and H’: magnified views of the PBI in (F and F’). K, L, K’, and L’: magnified views of the CHT in (I, J, I’, and J’). The number of embryos with similar gene expression patterns among all embryos examined was shown at the bottom right of each panel. The scale bars represent 250 μm. CHT, caudal hematopoietic tissue; HSPC, hematopoietic stem and progenitor cell; ICM, intermediate cell mass; PBI, posterior blood island; PRPF, pre-mRNA processing factor; WISH, whole-mount in situ hybridization.
|