FIGURE

Fig. 7

ID
ZDB-FIG-240319-30
Publication
Megerson et al., 2023 - Kremen1 regulates the regenerative capacity of support cells and mechanosensory hair cells in the zebrafish lateral line
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Fig. 7

Dorsoventral support cells contribute to supernumerary hair cells following regeneration in krm1nl10 mutant NMs (A) Timeline for Tg(sost:nlsEos)w215 photoconversion at 5dpf and homeostatic development until 8dpf. (B?C?) Homeostasis at 8 days of Tg(myosin6b:GFP)w186 labeled hair cells (green) and Tg(sost:nlsEos)w215 labeled dorsoventral support cells (magenta) following photoconversion in 5dpf heterozygous sibling and krm1nl10 mutant NMs. (D?F) Quantification of Tg(myosin6b:GFP)w186 hair cells, total Tg(sost:nlsEos)w215 expressing NM cells, and hair cells expressing both Tg(myosin6b:GFP)w186 and Tg(sost:nlsEos)w215 at 8dpf. Heterozygous siblings n = 21 NM (12 fish) and krm1nl10 n = 22 NM (12 fish). (G) Timeline for Tg(sost:nlsEos)w215 photoconversion and NEO at 5dpf and 1 round of regeneration until 8dpf. (H?I?) 1 round of regeneration of Tg(myosin6b:GFP)w186 labeled hair cells (green) and Tg(sost:nlsEos)w215 labeled dorsoventral support cells (magenta) following photoconversion at 5dpf and NEO-exposure in heterozygous sibling and krm1nl10 mutant NMs. (J?L) Quantification of Tg(myosin6b:GFP)w186 hair cells, total Tg(sost:nlsEos)w215 expressing NM cells, and hair cells expressing both Tg(myosin6b:GFP)w186 and Tg(sost:nlsEos)w215 at 8dpf following 1 round of regeneration. Heterozygous siblings n = 23 NM (8 fish) and krm1nl10 n = 31 NM (8 fish). (M) Timeline for Tg(sost:nlsEos)w215 photoconversion and NEO at 5dpf and again at 8dpf, for 2 rounds of regeneration until 11dpf. (N?O?) 2 rounds of regeneration of Tg(myosin6b:GFP)w186 labeled hair cells (green) and Tg(sost:nlsEos)w215 labeled dorsoventral support cells (magenta) following photoconversion and NEO-exposure at 5dpf and 8dpf in heterozygous sibling and krm1nl10 mutant NMs. (P?R) Quantification of Tg(myosin6b:GFP)w186 hair cells, total Tg(sost:nlsEos)w215 expressing NM cells, and hair cells expressing both Tg(myosin6b:GFP)w186 and Tg(sost:nlsEos)w215 at 8dpf following 2 rounds of regeneration. Heterozygous siblings n = 21 NM (8 fish) and krm1nl10 n = 22 NM (7 fish). All data presented as mean ± SD, Mann-Whitney test. Scale bar = 20 ?m.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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