Figure 5

The effect of amoxicillin pretreatment on hepatic steatosis induced by long-term fructose diet. (A) Schematic diagram of pretreatment with amoxicillin alone followed by later long-term glucose or fructose diet. (B) Quantification of hepatic steatosis by ORO staining in 10 dpf larvae subject to long-term glucose or fructose diet with amoxicillin pretreatment. The numbers of larvae analyzed in each group are indicated. In the Chi-square test, * and *** indicate p < 0.05, p < 0.001, and # p < 0.05, respectively. (C,D) Quantification of triglyceride and total cholesterol in 10 dpf larvae. 3 independent experiments. Mean + s.e.m. In the ANOVA analysis, ** indicates p < 0.01 (E) H&E staining of liver tissues at 10 dpf. Scale bar: 5 µm. (F–I) Quantification of the expression of pparα, acaca, fasn, and srebf1 at 10 dpf by real-time PCR. Four independent experiments. Mean + s.e.m. In the ANOVA analysis, * and ** indicate p < 0.05 and p < 0.01, respectively. dpf—days post-fertilization; AMX—amoxicillin; GLU—glucose; FRU—fructose.