FIGURE

Figure 5

ID

ZDB-FIG-240308-3

Publication

Sun et al., 2023 - Agaricus bisporus-Derived Glucosamine Hydrochloride Regulates VEGF through BMP Signaling to Promote Zebrafish Vascular Development and Impairment Repair

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

GAH repairs vascular impairment by upregulating the mRNA and protein levels of BMP and VEGF signaling pathway members. (a) Spatiotemporal expression of kdrl by ISH in different groups. Red arrows indicated the specific expression of kdrl in PMBC, PCV, CV, DA, and MCeV. Scale bars: 100 μm. The proportion of embryonic expression patterns in each group was shown as indicated and based on four (for DMH1) and three (for DM) repetitions. (b,d) Relative mRNA expression levels of bmp2b, bmp4, vegfaa, kdrl, and fli1 by quantitative PCR in different treatment groups as indicated. gapdh: reference gene. Each gene was tested at least five times. (c,e) Protein levels of Bmp2b, Bmp4, Vegfa, and Vegfr2 in different groups by ELISA assay. Each protein was repeated 2–3 times. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and NS, nonsignificant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Life (Basel)