Figure 3

GAH repairs vascular impairment by regulating the VEGF signal. (a1–d3) The vascular development of Tg(kdrl: mCherry) zebrafish at 72 hpf under a laser scanning confocal microscope. Zebrafish embryos were exposed to 0.15 mmol/L VRI to induce vascular impairment and then treated with GAH for recovery. Type 1~Type 3 indicated different vascular impairment phenotypes, from slight to severe. Arrows indicated: Se, intersegmental vessel; CCV, common cardinal vein; CV, caudal vein; MsV, mesencephalic vein; PCeV, posterior cerebral vein; CtA, central artery; PrA, prosencephalic artery; IOC, inner optic circle; AA3–AA6, first to fourth branchial arch; PHBC, primordial hindbrain channel; DLAV, dorsal longitudinal anastomotic vessel; CA, caudal artery. (a1–d1): diagram of whole-body blood vessels. (a2–d2): enlarged diagram of the blood vessels in the head. (a3–d3): enlarged diagram of intersegmental vessels. (e1,e2): classification criteria and statistics of different types for head and trunk. p < 0.001 (***). (f) Statistical data of different types of vascular phenotypes across different treatment groups. The experiment was repeated three times.