FIGURE

Fig. 5.

ID

ZDB-FIG-240307-35

Publication

Shangguan et al., 2024 - Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway

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Fig. 5.

The growth delay and chondrocyte malformation induced by kmt2d deficiency can be partially alleviated by the inhibition of Akt. a and b) The levels of the p-Akt (ser473) and p-β-catenin (ser552) proteins. The band density was quantified and expressed as the relative gray value (compared with the control) by arbitrarily setting the control value as 1. Data were pooled from 3 independent experiments, and the results were represented as mean ± SD. c) The reduced body length in kmt2d knockdowns was partially alleviated by ZSTK474. n = 10 for each group. d and e) The asymmetrical morphology ceratohyal and Meckel’s cartilage in kmt2d knockdowns was partially corrected. n = 10 for each group. Scale bar: d) 100 μm. *P < 0.05, **P < 0.001, ***P < 0.0001. Cb, ceratobranchial; Ch, ceratohyal; M, Meckel’s cartilage; Pq, palatoquadrate.

Expression Data

Antibodies:	Ab5-akt Ab24-ctnnb
Fish:	AB AB + CRISPR14-kmt2d + CRISPR15-kmt2d + CRISPR16-kmt2d
Knockdown Reagents:	CRISPR14-kmt2d CRISPR15-kmt2d CRISPR16-kmt2d
Anatomical Term:	whole organism
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR14-kmt2d + CRISPR15-kmt2d + CRISPR16-kmt2d
Condition:	chemical treatment by environment: ZSTK-474
Knockdown Reagents:	CRISPR14-kmt2d CRISPR15-kmt2d CRISPR16-kmt2d
Observed In:	ceratohyal cartilage Meckel's cartilage whole organism
Stage Range:	Shield to Day 5

Phenotype Detail

Acknowledgments

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